Loading health guide
Health Testing Guide
Which tests are mandatory, how to spot responsible breeders — and what it costs when things go wrong.
For the Neapolitan Mastiff we have compiled 4 mandatory and 3 recommended health tests, drawn from breed-club regulations, the University of PEI (CIDD), and OMIA — weighted by severity and heritability.
What this grade means
The Mastino Napoletano receives a grade of D because the breed is highly susceptible to severe joint conditions due to its massive size and extreme physical structure. While hip dysplasia (HD) and elbow dysplasia (ED) are mandatory health tests, the breed's extremely high body weight places massive, lifelong strain on the musculoskeletal system. Prospective buyers should also be prepared for a high risk of chronic joint degeneration and a relatively short life expectancy.
Every breed is susceptible to certain inherited conditions. Just like humans, dogs inherit predispositions from their parents. Responsible breeders screen their breeding dogs for these conditions and make informed mating decisions to reduce the risk passed on to puppies.
No — but it's the strongest tool we have. Diet, exercise, environment, and genetics all contribute to whether a dog develops a condition. When breeders use the right tests to identify risk in potential parents, the likelihood of many conditions appearing in the puppies drops significantly.
There's no one-size-fits-all here. For the Neapolitan Mastiff we track 4 mandatory tests plus 3 additional recommended ones. The specific tests and passing thresholds are detailed further below.
Keep in mind
Reliable screening tests still don't exist for many hereditary conditions, and tests for complex polygenic conditions are not always predictive of severity in the puppies. Even so, they're a powerful tool used by every responsible breeder.
There are two main types of health tests:
Screen the dog's DNA for known disease-causing mutations. Can be performed from puppyhood and give a binary result (clear / carrier / affected).
Clinical and imaging exams like X-ray, ultrasound, or ophthalmoscopy. They show how a predisposition actually manifests — usually only conclusive after 12–18 months.
We group breeding programs into three levels based on testing depth. Ask your breeder directly which level they meet.
A breeder at the Great level performs all mandatory tests plus 3 additional clinically recommended screenings that reduce risk in this breed.
In addition to mandatory tests:
Ectropion / Entropion
Augen
These are structural eyelid abnormalities affecting a dog's eyes. In cases of entropion, the eyelid rolls inward, causing the eyelashes or fur to rub painfully against the surface of the eyeball. In ectropion, the eyelid sags or rolls outward, leaving the sensitive inner lining of the eye exposed and unprotected.
Passing: CLEAR
Third eyelid (nictitating membrane) abnormalities - "cherry eye"
Augen und Sehvermögen
A prolapse of the nictitating membrane gland, commonly called 'cherry eye,' occurs when the tear gland of the third eyelid slips out of its normal position. This gland is responsible for producing a significant portion of the eye's protective tear film. When the connective tissue holding it in place weakens, the gland protrudes, appearing as a red, fleshy mass in the inner corner of the eye.
Physisch · Passing: CLEAR
Fold dermatitis/pyoderma
Haut und Fell
Fold dermatitis, also known as intertrigo, is an inflammatory skin condition that occurs within deep skin folds. When skin rubs against skin, it traps moisture, heat, and debris, creating an ideal environment for bacteria and yeast to overgrow. This leads to localized skin infections, particularly in breeds with pronounced facial, lip, or tail folds.
Physisch
These levels are a comparison framework, not an official certification. They help compare breeding programs at a glance.
A complete checklist with every mandatory and recommended test plus identity and rearing questions — take it with you.
Open the full checklistFor breeders
Machine-readable mating rules, minimum ages, scoring schemes, and what's required for HonestDog verification — as a printable guide.
Example rule — Elbow Dysplasia
Both parents must be ED-2 or better or better.
We compile mandatory tests from official breed regulations and veterinary databases — and check every uploaded certificate for authenticity.
L1 = mandatory under breed-club regulations. L2 = recommended or clinically indicated. L3 = lower prevalence. Every assignment carries a source and confidence rating.
VDH breeding regulations, CIDD (University of PEI), OMIA (University of Sydney), curated veterinary input.
Uploaded certificates are AI-extracted and reviewed by our team for authenticity, date, and result.
4 tests are currently mandatory under the relevant breed-club regulations. The most common are Elbow Dysplasia (ED), Hip dysplasia (HD), Dilated cardiomyopathy (DCM). Every test must be completed before mating, and the result must fall within the threshold defined by the regulation.
See breeders who have already uploaded their mandatory tests for verification.
View verified breeders