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Health Testing Guide
Which tests are mandatory, how to spot responsible breeders — and what it costs when things go wrong.
For the Markiesje we have compiled 3 mandatory and 2 recommended health tests, drawn from breed-club regulations, the University of PEI (CIDD), and OMIA — weighted by severity and heritability.
What this grade means
The Markiesje is considered a very vital breed, but due to the small population size, buyers must be mindful of hereditary risks. In particular, patellar luxation (dislocation of the kneecap) and progressive retinal atrophy (PRA), which leads to blindness, should be monitored by a veterinarian. Furthermore, the breed-specific Markiesje Paroxysmal Dyskinesia (MPD), a neurological movement disorder, is an important topic to address when searching for a puppy.
Every breed is susceptible to certain inherited conditions. Just like humans, dogs inherit predispositions from their parents. Responsible breeders screen their breeding dogs for these conditions and make informed mating decisions to reduce the risk passed on to puppies.
No — but it's the strongest tool we have. Diet, exercise, environment, and genetics all contribute to whether a dog develops a condition. When breeders use the right tests to identify risk in potential parents, the likelihood of many conditions appearing in the puppies drops significantly.
There's no one-size-fits-all here. For the Markiesje we track 3 mandatory tests plus 2 additional recommended ones. The specific tests and passing thresholds are detailed further below.
Keep in mind
Reliable screening tests still don't exist for many hereditary conditions, and tests for complex polygenic conditions are not always predictive of severity in the puppies. Even so, they're a powerful tool used by every responsible breeder.
There are two main types of health tests:
Screen the dog's DNA for known disease-causing mutations. Can be performed from puppyhood and give a binary result (clear / carrier / affected).
Clinical and imaging exams like X-ray, ultrasound, or ophthalmoscopy. They show how a predisposition actually manifests — usually only conclusive after 12–18 months.
We group breeding programs into three levels based on testing depth. Ask your breeder directly which level they meet.
A breeder at the Great level performs all mandatory tests plus 2 additional clinically recommended screenings that reduce risk in this breed.
In addition to mandatory tests:
Distichiasis
Augen und Sehvermögen
Distichiasis is an eye condition where extra eyelashes grow from the meibomian glands, which are the oil glands along the edge of the eyelid. These abnormal hairs point inward and rub directly against the cornea, the clear outer surface of the eye. This constant friction causes mechanical irritation and can damage the sensitive ocular surface.
Augen · Passing: Free or Mild
Cataract(HC)
Augen und Sehvermögen
A cataract is a progressive clouding of the lens inside the dog's eye, which is the clear structure that focuses light onto the retina at the back of the eye. When the lens becomes cloudy or opaque, it blocks light from entering, gradually impairing the dog's vision. If left untreated, this condition can eventually lead to complete blindness in the affected eye.
Augen · Passing: Free
These levels are a comparison framework, not an official certification. They help compare breeding programs at a glance.
A complete checklist with every mandatory and recommended test plus identity and rearing questions — take it with you.
Open the full checklistFor breeders
Machine-readable mating rules, minimum ages, scoring schemes, and what's required for HonestDog verification — as a printable guide.
Example rule — Progressive Retinal Atrophy (PRA)
Both parents must be Clear or Carrier or better.
We compile mandatory tests from official breed regulations and veterinary databases — and check every uploaded certificate for authenticity.
L1 = mandatory under breed-club regulations. L2 = recommended or clinically indicated. L3 = lower prevalence. Every assignment carries a source and confidence rating.
VDH breeding regulations, CIDD (University of PEI), OMIA (University of Sydney), curated veterinary input.
Uploaded certificates are AI-extracted and reviewed by our team for authenticity, date, and result.
3 tests are currently mandatory under the relevant breed-club regulations. The most common are Progressive Retinal Atrophy (PRA) (PRA), Patellar Luxation (PL), Paroxysmal Dyskinesia (PD). Every test must be completed before mating, and the result must fall within the threshold defined by the regulation.
See breeders who have already uploaded their mandatory tests for verification.
View verified breeders